高等生物物理技術
Advanced Biophysical Technology
| 節 | 週三 |
|---|---|
2 09:00–09:50 | 高等生物物理技術 BB106 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
從生物物理實驗技術入門,進而瞭解其所蘊藏的物理與生化原理。
物理,化學,生物
無備註
教師未提供此項資料
Midterm 30% Containing: test and experimental operation. Presentation and discuss 30% Final test 40%
教師未提供此項資料
| 週次 | 主題 |
|---|---|
| 第 1 週 | 1. Introduction. a. Factor affecting analyte structure and stability |
| 第 2 週 | b. Buffer system* c. Quantitation |
| 第 3 週 | 2. Hydrodynamic Methods a. Semi-permeable membrane* b. Viscosity* c. Thermal equilibrium dialysis* |
| 第 4 週 | d. Sedimentation* e. Micro-cone* |
| 第 5 週 | f. Flow cytometry g. Dynamic light scattering h. Static light scattering |
| 第 6 週 | 3. Chromatography a. Principle b. Equipment* c. Modes of chromatography* d. HPLC* e. Perfusion HPLC* |
| 第 7 週 | 4. Electrophoresis a. Principle b. DNA gel electrophoresis* c. RNA gel electrophoresis d. Protein electrophoresis* e. Two-dimensional electrophoresis* |
| 第 8 週 | 5. Spectroscopic Techniques a. Principle b. Electromagnetic (EM) spectrum c. UV/Vis absorption spectroscopy* d. Fluorescence spectroscopy |
| 第 9 週 | midterm |
| 第 10 週 | e. Circular dichroism (CD)* f. Infrared spectroscopy* g. Raman spectroscopy* h. NMR spectroscopy i. Electron spin resonance (ESR) spectroscopy |
| 第 11 週 | 6. Mass spectrometry a. Principle b. MS/MS c. LC/MS* d. GC/MS e. Eletrophoresis/MS |
| 第 12 週 | 7. Biocalorimetry a. Principle b. Isothermal titration calorimetry c. Differential scanning calorimetry |
| 第 13 週 | 8. Three-dimensional structure determination of macromolecules a. Structure determination by NMR b. X-ray diffraction by crystal c. Electron diffraction |
| 第 14 週 | 9. Computational Biology a. Molecular modeling b. MC/MD simulation |
| 第 15 週 | Specific topics presentation and discussion |
| 第 16 週 | Holiday |
| 第 17 週 | Specific topics presentation and discussion |
| 第 18 週 | Final test |
Physical Biochemistry: Principle and Applications.2nd edition Author: David Sheehan Publisher: John Wiley and sons, LTD (2009)
- 地點
- 生科實驗二館203室
- 時間
- 星期一早上09:00~12:00
- 聯絡方式
- x31633