高等生物技術導論
Advanced Biological Techniques
| 節 | 週四 |
|---|---|
1 08:00–08:50 | 高等生物技術導論 YD302（陽明） 2 節連堂 |
2 09:00–09:50 |
* 根據陽明交大上課時間表所列
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| 週次 | 主題 |
|---|---|
| 第 1 週 | Cell culture and bacterial culture techniques 2017/09/14 |
| 第 2 週 | Purification of plasmid DNA and genomic DNA 2017/09/21 |
| 第 3 週 | DNA fragment purification, agarose and polyacrylamide gel electrophoresisBasic concepts of biomaterials surface science 2017/09/28 |
| 第 4 週 | Basic concepts of biomaterials surface scienceDNA fragment purification, agarose and polyacrylamide gel electrophoresis 2017/10/05 |
| 第 5 週 | Restriction enzyme digestion and enzymatic manipulation of DNAPolymerase chain reaction, and Southern, Northern and Western blotting techniques 2017/10/12 |
| 第 6 週 | Polymerase chain reaction, and Southern, Northern and Western blotting techniquesReal-time PCR 2017/10/19 |
| 第 7 週 | Real-time PCRRestriction enzyme digestion and enzymatic manipulation of DNA 2017/10/26 |
| 第 8 週 | Introduction of DNA into bacteria and mammalian cells 2017/11/02 |
| 第 9 週 | 期中考 2017/11/09 |
| 第 10 週 | Transgenic and gene knock-out mice 2017/11/16 |
| 第 11 週 | Introduction to gene therapy 2017/11/23 |
| 第 12 週 | Adenoviral and retroviral DNA delivery (第一節: 黎萬君老師) The genome editing: CRISPR (第二節: 曾才郁老師)Adenoviral and retroviral DNA delivery 2017/11/30 |
| 第 13 週 | RNA interference 2017/12/07 |
| 第 14 週 | Genomic and proteomic studyGenomic and proteomic study; Immunoassay and immunoprecipitation 2017/12/14 |
| 第 15 週 | Immunoassay (ELISA; immunoperoxidase assay) and immunoprecipitationIn situ hybridization, immunohistochemistry, LCM and image analysis 2017/12/21 |
| 第 16 週 | In situ hybridization, immunohistochemistry, LCM and image analysis開國紀念日 2017/12/28 |
| 第 17 週 | Fluorescence-activated cell sorter (FACS) and confocal microscopy 2018/01/04 |
| 第 18 週 | 期末考 2018/01/11 |
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