生殖生物學實驗方法學

週次計畫

週次	主題
第 2 週	2/1 Lecture 1: Introduction – ovarian function & research background Laboratory Experiment: (1) Animal handling & Hormone injection & (2) preparation of culture wares 2018/02/27
第 3 週	2/2 Lecture 2: Methodology of cell culture Laboratory Experiment: Preparation of reagents & Coating culture plateswares / reagents 2018/03/06
第 4 週	2/3 Laboratory Experiment: Isolation of rat ovarian cells & Culture 2018/03/13
第 5 週	2/4 Laboratory Experiment: Change medium & Establishment of cell number standard curve 2/5 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs 2018/03/20
第 6 週	2/6 Lecture 3: Principle & application of gel electrophoresis 2/12 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs 2018/03/27
第 7 週	2/7 Laboratory Experiment: (1) Harvest of samples & Cell number determination assay, (2) Immunoblotting analysis (day1) 2018/04/03
第 8 週	2/8 Laboratory Experiment: Protein assay & Immunoblotting analysis (day2) 2018/04/10
第 9 週	2/9 Laboratory Experiment: Immunoblotting analysis (day 3) 2018/04/17
第 10 週	2/12 Lecture 4: Methodology of cell proliferation & apoptosis Laboratory Experiment: Zymography analysis (day 1) 2018/04/24
第 11 週	2/13 Laboratory Experiment: Zymography analysis (day 2) 2/14 Laboratory Experiment: Zymography analysis (day 3) 2018/05/01
第 12 週	2/23 Laboratory Experiment: Densitometric analysis-demonstration 2018/05/08
第 13 週	2/27 Lecture 5: Principle of enzyme immunoassays & nucleic analyses 2018/05/15
第 14 週	3/5 Laboratory Experiment: EIA- Establishment of standard curve (day 1) 3/6 Laboratory Experiment: EIA- Establishment of standard curve (day 2) & EIA- sample analysis (day 1) 2018/05/22
第 15 週	3/7 aboratory Experiment: EIA- sample analysis (day 2) 2018/05/29
第 16 週	3/13 Laboratory Experiment: Densitometric analysis of samples (1) 2018/06/05
第 17 週	3/20 Laboratory Experiment: Densitometric analysis of samples (2) 2018/06/12
第 18 週	3/27 Lecture 6: Data analysis & Discussion 2018/06/19