高等生物技術導論

週次計畫

週次	主題
第 1 週	Cell culture and bacterial culture techniques 2018/09/13
第 2 週	Purification of plasmid DNA and genomic DNA 2018/09/20
第 3 週	DNA fragment purification, agarose and polyacrylamide gel electrophoresisBasic concepts of biomaterials surface science 2018/09/27
第 4 週	Restriction enzyme digestion and enzymatic manipulation of DNADNA fragment purification, agarose and polyacrylamide gel electrophoresis 2018/10/04
第 5 週	Introduction of DNA into bacteria and mammalian cellsPolymerase chain reaction, and Southern, Northern and Western blotting techniques 2018/10/11
第 6 週	Polymerase chain reaction, and Southern, Northern and Western blotting techniquesReal-time PCR 2018/10/18
第 7 週	Real-time PCRRestriction enzyme digestion and enzymatic manipulation of DNA 2018/10/25
第 8 週	Basic concepts of biomaterials surface scienceIntroduction of DNA into bacteria and mammalian cells 2018/11/01
第 9 週	期中考 2018/11/08
第 10 週	Transgenic and gene knock-out mice 2018/11/15
第 11 週	Introduction to gene therapy 2018/11/22
第 12 週	Adenoviral and retroviral DNA delivery (第一節: 黎萬君老師) The genome editing: CRISPR (第二節: 曾才郁老師)Adenoviral and retroviral DNA delivery 2018/11/29
第 13 週	RNA interference 2018/12/06
第 14 週	Genomic and proteomic studyGenomic and proteomic study; Immunoassay and immunoprecipitation 2018/12/13
第 15 週	Immunoassay (ELISA; immunoperoxidase assay) and immunoprecipitationIn situ hybridization, immunohistochemistry, LCM and image analysis 2018/12/20
第 16 週	Fluorescence-activated cell sorter (FACS) and confocal microscopy開國紀念日 2018/12/27
第 17 週	In situ hybridization, immunohistochemistry, LCM and image analysisFluorescence-activated cell sorter (FACS) and confocal microscopy 2019/01/03
第 18 週	期末考 2019/01/10