生殖生物學實驗方法學
Experimental Methodology for Reproductive Biology
| 節 | 週二 |
|---|---|
3 10:10–11:00 | 生殖生物學實驗方法學 YR401（陽明） 2 節連堂 |
4 11:10–12:00 | |
5 13:20–14:10 | 生殖生物學實驗方法學 YR401（陽明） |
* 根據陽明交大上課時間表所列
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| 週次 | 主題 |
|---|---|
| 第 1 週 | 8/5 Lecture 1: Introduction – ovarian function & research background 2019/09/10 |
| 第 2 週 | 8/6 Lecture 2: Methodology of cell culture Laboratory Experiment: Introduction of experimental animal facility, animal care and behavior 2019/09/17 |
| 第 3 週 | 8/7 Lecture 3: Methodology of cell proliferation & apoptosis Laboratory Experiment: Lab orientation & Preparation of culture wares / reagents 2019/09/24 |
| 第 4 週 | 8/9 Laboratory Experiment: Animal handling & Hormone injection 8/10 Laboratory Experiment: Coating culture plates 2019/10/01 |
| 第 5 週 | 8/11 Laboratory Experiment: Isolation of rat ovarian cells & Culture 2019/10/08 |
| 第 6 週 | 8/12 Laboratory Experiment: Change medium & Establishment of cell number standard curve 8/13 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs 2019/10/15 |
| 第 7 週 | 8/15 Laboratory Experiment: Harvest of samples & Determination assays of cell number and protein content 2019/10/22 |
| 第 8 週 | 8/16 Lecture 4: Principle & application of gel electrophoresis 2019/10/29 |
| 第 9 週 | 8/19 Laboratory Experiment: Immunoblotting analysis (day1) 2019/11/05 |
| 第 10 週 | 8/20 Laboratory Experiment: Immunoblotting analysis (day 2) 2019/11/12 |
| 第 11 週 | 8/21 Laboratory Experiment: Immunoblotting analysis (day 3) 2019/11/19 |
| 第 12 週 | 8/22 Laboratory Experiment: Densitometric analysis-demonstration & practice 2019/11/26 |
| 第 13 週 | 8/23 Lecture 5: Principle of enzyme immunoassays & nucleic acid analyses 2019/12/03 |
| 第 14 週 | 8/26 Laboratory Experiment: Zymography analysis (day 1) 2019/12/10 |
| 第 15 週 | 8/27 Laboratory Experiment: Zymography analysis (day 2) 2019/12/17 |
| 第 16 週 | 8/28 Laboratory Experiment: Zymography analysis (day 3) 2019/12/24 |
| 第 17 週 | 8/29 Laboratory Experiment: Densitometric analysis of samples 2019/12/31 |
| 第 18 週 | 8/30 Lecture 6: Data analysis & Discussion 2020/01/07 |
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