高等生物技術導論

週次計畫

週次	主題
第 1 週	Cell culture and bacterial culture techniques 2019/09/12
第 2 週	Purification of DNA and gel electrophoresisPurification of plasmid DNA and genomic DNA 2019/09/19
第 3 週	Restriction enzyme digestion and enzymatic manipulation of DNABasic concepts of biomaterials surface science 2019/09/26
第 4 週	Basic concepts of biomaterials surface scienceDNA fragment purification, agarose and polyacrylamide gel electrophoresis 2019/10/03
第 5 週	國慶日(放假一天)Polymerase chain reaction, and Southern, Northern and Western blotting techniques 2019/10/10
第 6 週	Polymerase chain reaction, and Southern, Northern and Western blotting techniquesReal-time PCR 2019/10/17
第 7 週	Real-time PCRRestriction enzyme digestion and enzymatic manipulation of DNA 2019/10/24
第 8 週	Introduction of DNA into bacteria and mammalian cells 2019/10/31
第 9 週	期中考 2019/11/07
第 10 週	Transgenic and gene knock-out mice 2019/11/14
第 11 週	Introduction to gene therapy 2019/11/21
第 12 週	Adenoviral and retroviral DNA delivery (第一節: 黎萬君老師) The genome editing: CRISPR (第二節: 曾才郁老師)Adenoviral and retroviral DNA delivery 2019/11/28
第 13 週	RNA interference 2019/12/05
第 14 週	Genomic and proteomic studyGenomic and proteomic study; Immunoassay and immunoprecipitation 2019/12/12
第 15 週	Immunoassay (ELISA; immunoperoxidase assay) and immunoprecipitationIn situ hybridization, immunohistochemistry, LCM and image analysis 2019/12/19
第 16 週	In situ hybridization, immunohistochemistry, LCM and image analysis開國紀念日 2019/12/26
第 17 週	Fluorescence-activated cell sorter (FACS) and confocal microscopy 2020/01/02
第 18 週	期末考 2020/01/09