生物光譜學
Biospectroscopy
| 節 | 週四 |
|---|---|
5 13:20–14:10 | 生物光譜學 BI309（博愛） 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
詳細概論光學光譜理論‚並介紹如何將這些理論運用於現代分子和細胞生物物理和生物化學◦內容包括分子中電子能階與轉動能階的吸收,螢光光譜的形成與運用,圓二色性的原理與應用,核磁共振原理與圖譜解析與X-ray繞射結晶學等應用◦ This course will provide fundamental concepts on optical spectroscopy which is used to study the structure and function of biological macromolecules. The goal of this course is to help students to develop physical understanding about daily-used instruments in biophysical and biochemistry laboratory.
基礎物理、基礎化學 (General physics and chemistry) Although all the course materials are prepared in English, this course is still a Mandarin-orientated course. All the lectures will be given in Mandarin.
無備註
Lecture/ PowerPoint slides:
1學期作業:佔總學期30分(約5份) (5 sets of homework; 30% of total final score) 2.考試狀況:期中考與期末考各佔佔總學期35分 (one midterm and one final exam; 35% of total final score for each exam ) 3.評量方法 Home Wrok:30%; Exams: 70% (35% for each exam)
Absorption spectroscopy
1. Basic principles 2. Absorption spectroscopy of electronic states 3. Spectroscopic analysis of biopolymers 4. Effects of conformation on absorption 5. Circular dichroism
- 講授：
- 6
Fluorescence spectroscopy
1. Basic principles 2. Factors governing of fluorescence intensity 3. Polarization and anisotropy 4. Energy transfer
- 講授：
- 6
Infrared and Raman spectroscopy
Principles of infrared spectroscopy 2. Vibrational spectra of biopolymers 3. Raman spectroscopy
- 講授：
- 4
Nuclear magnetic spectroscopy
1. Basic principles 2. Feature of NMR spectra 3. NMR spectra of biological systems 4.General principles of electron paramagnetic resonance
- 講授：
- 4
X-ray crystallography
1. X-ray scattering and diffraction 2. Properties of crystals 3. Determination of molecular structure by X-ray crystallography 4. Determining the structure of a macromolecule 5.more scattering and diffraction techniques
- 講授：
- 4
Class discussion/ presentation
Case studies
- 講授：
- 4
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction; Light & Matters 3/5 |
| 第 2 週 | Light and Matters - Quantum Chemistry point of view 3/12 |
| 第 3 週 | Absorption spectroscopy I: Fundamental 3/19 |
| 第 4 週 | Absorption spectroscopy II: Instrumentation and application 3/26 |
| 第 5 週 | National holiday 4/2 |
| 第 6 週 | Fluorescence spectroscopy: I. Fundamental 4/9 |
| 第 7 週 | Fluorescence spectroscopy II: Instrumentation and application 4/16 |
| 第 8 週 | Fluorescence spectroscopy III: Anisotropy and FRET 4/23 |
| 第 9 週 | Infrared and Raman spectroscopy I: Fundamental 4/30 |
| 第 10 週 | Midterm 5/7 |
| 第 11 週 | Infrared and Raman spectroscopy II: Instrumentation and application 5/14 |
| 第 12 週 | Nuclear magnetic spectroscopy I: Fundamental 5/21 |
| 第 13 週 | Nuclear magnetic spectroscopy II: Instrumentation and application 5/28 |
| 第 14 週 | X-ray crystallography I: Fundamental 6/4 |
| 第 15 週 | X-ray crystallography II: application 6/11 |
| 第 16 週 | Case studies 6/18 |
| 第 17 週 | National holiday 6/25 |
| 第 18 週 | Final exam (Open Book exam) 7/2 |
‧Charles R. Cantor and Paul R. Scimmel, Biophysical chemistry part II: Techniques for the study of biological structure and function, 1st Ed., W. H. Freeman &Co Ltd., 1980 ‧ Joseph. R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., New York : Springer, 2006 ‧ Bernard Valeur, Molecular fluorescence, 1st Ed., Willey-VCH, 2002 ‧ Daniel C. Harris and Michael D. Bertoluccim Symmetry and Spectroscopy: An Introduction to Vibrational and Electronic Spectroscopy, 1989 ‧ by Robert M. Silverstein, Francis X. Webster, and David Kiemle, Spectrometric Identification of Organic Compounds, ; 7th Ed., John Wiley & Sons, 2005 ‧ Albrecht Messerschmidt, X-ray crystallography of biomacromolecules: a practical guide, 1st Ed., Willey-VCH, 2007
- 地點
- 生科系 實驗一館111室
- 時間
- 週三 8:00~10:00
- 聯絡方式
- yatingkao@nctu.edu.tw