光學生物影像技術
Optical Bio-imaging
| 節 | 週四 |
|---|---|
2 09:00–09:50 | 光學生物影像技術 BI308（博愛） 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
介紹現有光學生物影像技術 目標: (1) To provide fundamental concepts in optics, laser, microscopy, linear and nonlinear molecular spectroscopy. (2) To help students develop physical understanding about the underlying principles of various emerging optical imaging techniques. (3) To motivate individuals into this exciting field to make innovation
基礎物理、基礎化學
無備註
As discussed earlier, this course would be adjusted to a "web-based" and "online-teaching" course. All materials will be uploaded and you will find youtube linkages when you click the "view,檢視" button. You can copy and paste those links as URLs. Lecture/ PowerPoint slides: PowerPoint slides will be uploaded to the course website one week before the corresponding lectures.
Q&A discussions on Wednesdays will be graded. (70%) Q&A Homework. (30%)
Spectroscopy
1.liner molecule spectroscopy 2. Fluorescence spectroscopy 3. Nonlinear molecular spectroscopy
- 講授：
- 9
Microscopy
1. Basic light microscopy 2. Confocal microscopy 3. Total internal reflection; 4. Sheet illumination 5.Multi-photon fluorescence imaging
- 講授：
- 9
Optical probes
1. Dye molecules 2. GFP-like-protein 3. fluorescent high lighter
- 講授：
- 6
Super-resolution
1. STED 2. PALM 3.SSIM
- 講授：
- 3
Applications
1. Single-molecule biophysics 2. Label-free chemical imaging
- 講授：
- 6
Class discussion/ presentation
1. Presentation I 2. Presentation I 3. Presentation I
- 實作：
- 9
| 週次 | 主題 |
|---|---|
| 第 1 週 | Course introduction; Syllabus; Light and matter; Optics 3/5 |
| 第 2 週 | Optics components 3/12 |
| 第 3 週 | Linear molecular spectroscopy 3/19 |
| 第 4 週 | Fluorescence spectroscopy 3/26 |
| 第 5 週 | National Holiday 4/2 |
| 第 6 週 | Nonlinear molecular spectroscopy, Imaging probes (fluorescence dye fluorescent protein Quantum dot) 4/9 |
| 第 7 週 | Basic light microscopy; Confocal microscopy; Multi-photon fluorescent microscopy 4/16 |
| 第 8 週 | Total internal reflection microscopy; Light-sheet illumination 4/23 |
| 第 9 週 | Penetration-depth improvement; CLARITY 4/30 |
| 第 10 週 | Super-resolution imaging 5/7 |
| 第 11 週 | Force microscopy 5/14 |
| 第 12 週 | Label-free chemical imaging 5/21 |
| 第 13 週 | Single-molecule biophysics 5/28 |
| 第 14 週 | Fast imaging 6/4 |
| 第 15 週 | Emerging technology 6/11 |
| 第 16 週 | Student presentations and discussion 6/18 |
| 第 17 週 | National Holiday 6/25 |
| 第 18 週 | Term paper hand in and discussions 7/2 |
以下均為參考書目: ‧ P. N. Prasad, Introduction to Biophotonics, 1st Ed., New York : Wiley, 2003 ‧ J. R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., New York : Springer, 2006 ‧ J. Pawley (ed), Handbook of biological confocal microscopy, 2nd Ed., New York : Springer, 1995 ‧ R. W. Boyd, Nonlinear optics 3rd Ed., Boston: Academic Press, 2008 ‧ B. R. Masters and P. So, Handbook of biomedical nonlinear optical microscopy, 1st Ed., New York: Oxford University Press, 2008 ‧Rafael Yuste (ed.), Imaging: a laboratory manual, 1st Ed., New York: Cold Spring Harbor Laboratory, 2010.
- 地點
- 博愛校區 實驗一館111室
- 時間
- 週三 9:00~12:00 am
- 聯絡方式
- yatingkao @nctu.edu.tw