光學顯微技術

週次計畫

週次	主題
第 1 週	緒論: 光學與成像原理Introduction: Optics and Imaging 2020/03/06
第 2 週	光學顯微鏡基本構造Basic components of a light microscope 2020/03/13
第 3 週	常用光學顯微鏡技術: Bright field, Dark field, Rheinberg illumination, Phase contrastTechniques in microscopy: Bright field, Dark field, Rheinberg illumination, Phase contrast 2020/03/20
第 4 週	DIC顯微鏡與實習Differential interference contrast (DIC) microscopy 2020/03/27
第 5 週	螢光顯微鏡與實習Fluorescence microscopy and lab 2020/04/03
第 6 週	共軛焦顯微鏡與實習Confocal microscopy and lab 2020/04/10
第 7 週	清明節Development of fluorescent proteins and probes 2020/04/17
第 8 週	螢光蛋白與分子螢光探針之發展Multi-photon microscopy and lab 2020/04/24
第 9 週	多光子顯微鏡與實習Midterm exam 2020/05/01
第 10 週	期中考Spectral imaging, FRAP, FLIP, and FSM 2020/05/08
第 11 週	高等螢光顯微鏡:Spectral imaging, FRAP, FLIP, and FSMLive cell imaging and deconvolution microscopy 2020/05/15
第 12 週	活細胞顯微系統與Deconvolution microscopyFRET, FLIM, and Optical tweezers 2020/05/22
第 13 週	特殊光學顯微鏡:FRET, FLIMTotal internal reflection fluorescence (TIRF) microscopy, light-sheet microscopy, and Fluorescence correlation spectroscopy (FCS) 2020/05/29
第 14 週	特殊光學顯微鏡:Optical tweezers, Total internal reflection fluorescence (TIRF) microscopySuperresolution Microscopy: Near-field scanning optical microscope (NSOM), Stochastic optical reconstruction microscopy (STORM), Photoactivated localization microscopy (PALM), Stimulated emission depletion (STED) microscopy 2020/06/05
第 15 週	特殊光學顯微鏡:light-sheet microscopy, and Fluorescence correlation spectroscopy (FCS)Structured illumination microscopy (SIM), Super-resolution Optical Fluctuation Imaging (SOFI) 2020/06/12
第 16 週	超解像力顯微鏡:Near-field scanning optical microscope (NSOM), Stochastic optical reconstruction microscopy (STORM), Photoactivated localization microscopy (PALM), Stimulated emission depletion (STED) microscopyStudent presentation 2020/06/19
第 17 週	期末報告Student presentation 2020/06/26
第 18 週	期末考Final exam 2020/07/03