生殖生物學實驗方法學
Experimental Methodology for Reproductive Biology
| 節 | 週二 |
|---|---|
3 10:10–11:00 | 生殖生物學實驗方法學 YR401（陽明） 2 節連堂 |
4 11:10–12:00 | |
5 13:20–14:10 | 生殖生物學實驗方法學 YR401（陽明） |
* 根據陽明交大上課時間表所列
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| 週次 | 主題 |
|---|---|
| 第 1 週 | 8/3-Lecture 1: Introduction – ovarian function & research background 2020/09/15 |
| 第 2 週 | 8/4-Lecture 2: Methodology of animal care & behavior, and cell culture Laboratory Experiment: Introduction of experimental animal facility, and animal care & behavior 2020/09/22 |
| 第 3 週 | 8/5-Lecture 3: Methodology of cell proliferation, and determination of cell viability and DNA content Laboratory Experiment: Lab orientation & Preparation of culture wares / reagents 2020/09/29 |
| 第 4 週 | 8/7-Laboratory Experiment: Animal handling & Hormone injection 2020/10/06 |
| 第 5 週 | 8/8-Laboratory Experiment: Coating culture plates 2020/10/13 |
| 第 6 週 | 8/9-Laboratory Experiment: Isolation of rat ovarian cells & Culture 2020/10/20 |
| 第 7 週 | 8/10-Laboratory Experiment: Change medium & Establishment of cell number standard curve 2020/10/27 |
| 第 8 週 | 8/11-Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs 2020/11/03 |
| 第 9 週 | 8/13-Laboratory Experiment: Harvest of samples & Determination assays of cell number and protein content 2020/11/10 |
| 第 10 週 | 8/17-Lecture 4: Principle & application of gel electrophoresis Laboratory Experiment: Immunoblotting analysis (day1) 2020/11/17 |
| 第 11 週 | 8/18-Laboratory Experiment: Immunoblotting analysis (day 2) 2020/11/24 |
| 第 12 週 | 8/19-Laboratory Experiment: Immunoblotting analysis (day 3) 2020/12/01 |
| 第 13 週 | 8/20-Laboratory Experiment: Densitometric analysis demonstration & practice 2020/12/08 |
| 第 14 週 | 8/21-Laboratory Experiment: Densitometric analysis of samples 2020/12/15 |
| 第 15 週 | 8/24-Lecture 5: Principle of enzyme immunoassays 2020/12/22 |
| 第 16 週 | 8/25-Laboratory Experiment: Enzyme immunoassay (day 1) 2020/12/29 |
| 第 17 週 | 8/26-Laboratory Experiment: Enzyme immunoassay (day 2) 2021/01/05 |
| 第 18 週 | 8/31-Lecture 6: Data analysis & Discussion 2021/01/12 |
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