生物影像分析在細胞生物學的應用

週次計畫

週次	主題
第 1 週	Introduction 2020/09/17
第 2 週	Basic light microscopy 1. Introduction 2. Light and optics 3. Phase contrast 4. DIC 5. Darkfield 2020/09/24
第 3 週	Fluorescence microscopy (I) 1. Fluorescence basic 2. Fluorescence probes 3. Fluorescence optics 4. Epi-microscopy 2020/10/01
第 4 週	Fluorescence microscopy (II) 1. Confocal fluorescence microscopy 2. TIRFM 3. TIRFM for exocytosis and endocytosis 2020/10/08
第 5 週	Biological applications of fluorescence microscopy 1. Ratio imaging for [Ca2+]i 2. Ratio imaging for pH 3. FRAP/FLIP 2020/10/15
第 6 週	Intravital microscopy 2020/10/22
第 7 週	Superresolution microscopy 2020/10/29
第 8 週	Advanced fluorescence microscopy (I) FRET/Fluorescence lifetime imaging 2020/11/05
第 9 週	Advanced fluorescence microscopy (II) FCS for molecular dynamics 2020/11/12
第 10 週	Advanced fluorescence microscopy (III) Nonlinear and other light microscopy 2020/11/19
第 11 週	Electron microscopy, Scanning probe microscopy 2020/11/26
第 12 週	Digital imaging, image processing and feature extraction 2020/12/03
第 13 週	Lab rotaion: Lab (I) Basic optics alignment, phase contrast and DIC micorscopy-lab (pm1:30-4:30) Lab (II) Confocal microcopy-lab (pm1:30-4:30) Lab (III) TIRFM and Spining-disc confocal microcopy (pm1:30-4:30) 2020/12/10
第 14 週	Lab rotaion: Lab (I) Basic optics alignment, phase contrast and DIC micorscopy-lab (pm1:30-4:30) Lab (II) Confocal microcopy-lab (pm1:30-4:30) Lab (III) TIRFM and Spining-disc confocal microcopy (pm1:30-4:30) 2020/12/17
第 15 週	Lab rotaion: Lab (I) Basic optics alignment, phase contrast and DIC micorscopy-lab (pm1:30-4:30) Lab (II) Confocal microcopy-lab (pm1:30-4:30) Lab (III) TIRFM and Spining-disc confocal microcopy (pm1:30-4:30) 2020/12/24
第 16 週	Microscopy-based data mining: ImageJ, CellProfiler and Weka 2020/12/31
第 17 週	Lab (IV) Numerical morphological analysis of 3D mitochondria morphology by microP3D 2021/01/07
第 18 週	Lab (V) Profiling drug effects on mitochondrial and chromosomal partition by high content analysis 2021/01/14