高等生物技術導論

週次計畫

週次	主題
第 1 週	Cell culture and bacterial culture techniques 2020/09/17
第 2 週	Purification of DNA and gel electrophoresis Restriction enzyme digestion and enzymatic manipulation of DNAPurification of plasmid DNA and genomic DNA 2020/09/24
第 3 週	中秋節(放假一天)Basic concepts of biomaterials surface science 2020/10/01
第 4 週	Introduction of DNA into bacteria and mammalian cells DNA fragment purification, agarose and polyacrylamide gel electrophoresis 2020/10/08
第 5 週	Basic concepts of biomaterials surface sciencePolymerase chain reaction, and Southern, Northern and Western blotting techniques 2020/10/15
第 6 週	Polymerase chain reaction, and Southern, Northern and Western blotting techniquesReal-time PCR 2020/10/22
第 7 週	Real-time PCRRestriction enzyme digestion and enzymatic manipulation of DNA 2020/10/29
第 8 週	期中考Introduction of DNA into bacteria and mammalian cells 2020/11/05
第 9 週	Transgenic and gene knock-out mice期中考 2020/11/12
第 10 週	Introduction to gene therapyTransgenic and gene knock-out mice 2020/11/19
第 11 週	Adenoviral and retroviral DNA delivery (第一節: 黎萬君老師) The genome editing: CRISPR (第二節: 曾才郁老師)Introduction to gene therapy 2020/11/26
第 12 週	RNA interferenceAdenoviral and retroviral DNA delivery 2020/12/03
第 13 週	Genomic and proteomic studyRNA interference 2020/12/10
第 14 週	Immunoassay (ELISA; immunoperoxidase assay) and immunoprecipitationGenomic and proteomic study; Immunoassay and immunoprecipitation 2020/12/17
第 15 週	In situ hybridization, immunohistochemistry, LCM and image analysis 2020/12/24
第 16 週	期末考開國紀念日 2020/12/31
第 17 週	練習規劃實驗步驟及藥品配製(作業繳交日期2020/10/08)Fluorescence-activated cell sorter (FACS) and confocal microscopy 2021/01/07
第 18 週	Fluorescence-activated cell sorter (FACS) and confocal microscopy (on line)(期末考範圍)期末考 2021/01/14