光學生物影像技術
Optical Bio-imaging
| 節 | 週四 |
|---|---|
2 09:00–09:50 | 光學生物影像技術 BI308（博愛） 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
介紹現有光學生物影像技術 目標: (1) To provide fundamental concepts in optics, laser, microscopy, linear and nonlinear molecular spectroscopy. (2) To help students develop physical understanding about the underlying principles of various emerging optical imaging techniques. (3) To motivate individuals into this exciting field to make innovation
基礎物理、基礎化學
無備註
Lecture/ PowerPoint slides: PowerPoint slides will be uploaded to the course website one week before the corresponding lectures.
Homework: 30% (3~5 problem-set) Take-home midterm (35%) Term paper :35% Pick up one of the topics in which you have the most interest and write a review-type term paper (7~12 pages; 12 pt; 1.5 line spacing) on the principles of a particular optical imaging method and its applications to biological studies. More than 30 references are required. Term paper types: (choose one type) (1) For methodology development: (a)Historical view: why this new method is needed (b)Concept original (c)Comparison with the previous method (d)Possible further application (2)For a biological system: (a) Which biological system had been investigated? What is its significance? (b) Which optical probing method is used? (c) Comparison with other methods used in studying this biological system (d) New finding by using this optical probing method
Spectroscopy
1.liner molecule spectroscopy 2. Fluorescence spectroscopy 3. Nonlinear molecular spectroscopy
- 講授：
- 9
Microscopy
1. Basic light microscopy 2. Confocal microscopy 3. Total internal reflection; 4. Sheet illumination 5.Multi-photon fluorescence imaging
- 講授：
- 9
Optical probes
1. Dye molecules 2. GFP-like-protein 3. fluorescent high lighter
- 講授：
- 6
Super-resolution
1. STED 2. PALM 3.SSIM
- 講授：
- 3
Applications
1. Single-molecule biophysics 2. Label-free chemical imaging
- 講授：
- 6
Class discussion/ presentation
1. Presentation I 2. Presentation I 3. Presentation I
- 實作：
- 9
| 週次 | 主題 |
|---|---|
| 第 1 週 | Course introduction; Syllabus; Light and matter; Optics 2/25 |
| 第 2 週 | Optics components 3/18 |
| 第 3 週 | Linear molecular spectroscopy 3/11 |
| 第 4 週 | Fluorescence spectroscopy 3/18 |
| 第 5 週 | Nonlinear molecular spectroscopy, 3/25 |
| 第 6 週 | Imaging probes (fluorescence dye fluorescent protein Quantum dot) 4/1 |
| 第 7 週 | Basic light microscopy; Confocal microscopy; Multi-photon fluorescent microscopy 4/8 |
| 第 8 週 | Total internal reflection microscopy; Light-sheet illumination 4/15 |
| 第 9 週 | Penetration-depth improvement; CLARITY 4/22 |
| 第 10 週 | Super-resolution imaging 4/29 |
| 第 11 週 | Force microscopy 5/6 |
| 第 12 週 | Label-free chemical imaging 5/13 |
| 第 13 週 | Single-molecule biophysics 5/20 |
| 第 14 週 | Fast imaging 5/27 |
| 第 15 週 | Emerging technology 6/3 |
| 第 16 週 | Emerging technology 6/10 |
| 第 17 週 | Term paper discussions 6/17 |
| 第 18 週 | Term paper discussions 6/24 |
以下均為參考書目: ‧ P. N. Prasad, Introduction to Biophotonics, 1st Ed., New York : Wiley, 2003 ‧ J. R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., New York : Springer, 2006 ‧ J. Pawley (ed), Handbook of biological confocal microscopy, 2nd Ed., New York : Springer, 1995 ‧ R. W. Boyd, Nonlinear optics 3rd Ed., Boston: Academic Press, 2008 ‧ B. R. Masters and P. So, Handbook of biomedical nonlinear optical microscopy, 1st Ed., New York: Oxford University Press, 2008 ‧Rafael Yuste (ed.), Imaging: a laboratory manual, 1st Ed., New York: Cold Spring Harbor Laboratory, 2010.
- 地點
- 博愛校區賢齊館424室
- 時間
- 週三 9:00~12:00 am
- 聯絡方式
- yatingkao @nctu.edu.tw