遺傳學原理的實作與應用
Laboratory in Fundamental Genetics and its Applications
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 遺傳學原理的實作與應用 4 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 | |
8 16:30–17:20 |
* 根據陽明交大上課時間表所列
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無備註
實驗課程對於對於大學生進入生命科學領域來說至關重要,然而許多傳統實驗課程設計往往與真正研究脫節,因此對於吸引學生與幫助學生踏入研究領域的幫助往往有限,為了讓提昇實驗課對於學生的幫助,本課將透過模擬真正研究進程來規畫整學期之課程主軸,並透過小組教學模擬實驗室的分工合作與促進組員間的腦力激盪,最後透過多方面評量來給出學生最終成績,期望能讓學生了解真正的研究是如何來進行,且提高學生進入研究領域的興趣。大學的生命科學知識和高中最大的不同在於課堂所學知識將不再僅限於教科書上的書寫,大學生將有機會自己參與在生命科學的研究領域。以本課程「酵母菌遺傳學法則」為例,基本的遺傳學概念如孟德爾遺傳學法則在學生們高中時期就有所接觸,但是如何透過實際實驗來去證明並歸納出遺傳法則的概念相當的薄弱,這基本是源自於學生缺乏直接透過實驗證明孟德爾遺傳學法則的機會,因此我們將課程設計成模擬當初孟德爾的研究進程,期望能以此加深學生對於遺傳學的理解並讓學生能夠了解一個完整研究的進行過程。Mendelian inheritance has widely been considered as a fundamental genetics concept in the modern biology classroom. With a growing awareness that contemporary genetics is broader in content, we aim to implement instructional techniques based on educational research on active learning and backward design. The course will introduce students to molecular genetics and synthetic biology within the context of semester-long research projects in Mendel’s principles. Each course project includes both theoretical background material as well as detailed yeast experimental procedures. All of the experiments have been thoroughly tested with a senior group of students serving roles as teaching assistants. Future courses can benefit from wider adoption of this project, more emphasis on the biological design of curricula as a science, and new methods of assessing student development on research career.
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| 週次 | 主題 |
|---|---|
| 第 1 週 | Yeast as a genetic model 2022/02/18 |
| 第 2 週 | Yeast genetics – Getting started with yeast 2022/02/25 |
| 第 3 週 | Cell cycle of yeast – Culture and growth curve 2022/03/04 |
| 第 4 週 | Growth and manipulation of yeast – Genotype and phenotype 2022/03/11 |
| 第 5 週 | Growth and manipulation of yeast – Auxotroph and its implication in yeast genetics 2022/03/18 |
| 第 6 週 | Characterization of yeast mutants – Genetic complementation 2022/03/25 |
| 第 7 週 | Assay of yeast mating-type switches 2022/04/01 |
| 第 8 週 | Yeast meiosis and sporulation 2022/04/08 |
| 第 9 週 | Tetrad dissection and analysis 2022/04/15 |
| 第 10 週 | Mapping of yeast chromosomes 2022/04/22 |
| 第 11 週 | Gene linkage using meiotic mapping 2022/04/29 |
| 第 12 週 | Analysis of chromosome segregation in yeast 2022/05/06 |
| 第 13 週 | Epistasis analysis 2022/05/13 |
| 第 14 週 | Suppressor mutation – ade2 suppressor screening 2022/05/20 |
| 第 15 週 | Gene and gene interactions – synthetic lethality 2022/05/27 |
| 第 16 週 | Modern techniques for recombinant DNA assembly 2022/06/03 |
| 第 17 週 | Engineered and synthetic yeast – control and reprogram biological circuits 2022/06/10 |
| 第 18 週 | Final discussion and check-out 2022/06/17 |
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