基因操作及表現
Gene manipulation and expression
| 節 | 週四 |
|---|---|
5 13:20–14:10 | 基因操作及表現 YEC02 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
課程內容包括Fundamental techniques of gene manipulation, Plasmids, cosmids, and other vectors, Gel electrophoresis, Gene cloning strategies, Extraction, purification, and analysis of genomic DNA and mRNA from eukaryotic cells, Cloning in bacteria, yeast, and other fungi, PCR and DNA sequencing, Construction of genomic DNA and cDNA libraries, Analysis of gene expression, Mutagenesis and protein engineering, Gene transfer to animal cells, RNA interference, Gene targeting, CRISPR/Cas9 and targeted genome editing, Genetic manipulation of animals, Applications of recombinant DNA technology
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使學生具有基因操作及表現相關的基本知識,以及瞭解此技術的重要性、應用性,這些課程將有助於學生的論文研讀及研究工作。
筆試(期中與期末考)、課中討論、分組討論、書面報告
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| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction 2022/02/17 |
| 第 2 週 | Fundamental techniques of gene manipulation 2022/02/24 |
| 第 3 週 | Plasmids, cosmids, and other vectors 2022/03/03 |
| 第 4 週 | Gel electrophoresis 2022/03/10 |
| 第 5 週 | Gene cloning strategies 2022/03/17 |
| 第 6 週 | Extraction, purification, and analysis of genomic DNA and mRNA from eukaryotic cells 2022/03/24 |
| 第 7 週 | PCR and DNA sequencing 2022/03/31 |
| 第 8 週 | Mid-term exam 2022/04/07 |
| 第 9 週 | Cloning in bacteria, yeast, and other fungi 2022/04/14 |
| 第 10 週 | Construction of genomic DNA and cDNA libraries 2022/04/21 |
| 第 11 週 | Analysis of gene expression 2022/04/28 |
| 第 12 週 | Mutagenesis and protein engineering 2022/05/05 |
| 第 13 週 | Gene transfer to animal cells 2022/05/12 |
| 第 14 週 | RNA interference 2022/05/19 |
| 第 15 週 | CRISPR/Cas9 and targeted genome editing 2022/05/26 |
| 第 16 週 | Final exam 2022/06/02 |
| 第 17 週 | Gene targeting (自主學習) 2022/06/09 |
| 第 18 週 | Applications of recombinant DNA technology (自主學習) 2022/06/16 |
Principles of Gene Manipulation and Genomics: Principles and Applications of Recombinant DNA and Genomics; Richard M. Twyman and Sandy Primrose; Blackwell Pub., 8th ed., 2016
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