分子生物技術
Techniques of Molecular Biology
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 分子生物技術 YR105（陽明） 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
1. Nucleic acid isolation, electrophoresis, nucleic acid hybridization, Southern & Northern blotting 2. Restriction endonuclease and DNA cloning 3. PCR and DNA sequencing 4. Gene delivery techniques: Transformation, Transfection & Infection 5. Methods in reconstructing a phylogenetic tree using molecular data 6. Genomic approaches & bioinformatics 7. Genotyping 8. Protein overexpression, purification, and detection 9. Protein-protein interaction, nucleic acid-protein interactions and immunoprecipitation & ChIP 10. Proteomics approaches 11. Radiobioassay 12. Fluorescent microscopy and image 13. Flow cytometry 14. Metabolomics 15. Electrophysiological recording techniques
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無備註
1. 瞭解分子生物學技術之原理與操作 2. 瞭解分子生物學技術在轉譯醫學研究上的應用 3. 瞭解細胞生物學技術之原理與操作 4. 瞭解細胞生物學技術在轉譯醫學研究上的應用
1. 課中討論 2. 分組討論 3. 書面報告
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| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction 9/16 |
| 第 2 週 | PCR and DNA sequencingPCR and DNA sequencing 9/23 |
| 第 3 週 | Restriction endonuclease and DNA cloning 9/30 |
| 第 4 週 | Nucleic acid isolation, electrophoresis, nucleic acid hybridization, Southern & Northern blotting 10/7 |
| 第 5 週 | Gene delivery techniques: Transformation, transfection & infection 10/14 |
| 第 6 週 | Real-time quantitative PCR 10/21 |
| 第 7 週 | Genomic approaches 10/28 |
| 第 8 週 | Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation 11/4 |
| 第 9 週 | Mass spectrometry 11/11 |
| 第 10 週 | Methods in reconstructing a phylogenetic tree using molecular data 11/18 |
| 第 11 週 | Metabolomics 11/25 |
| 第 12 週 | Proteomics approaches 12/2 |
| 第 13 週 | Fluorescence imaging 12/9 |
| 第 14 週 | Protein overexpression, purification, and detection 12/16 |
| 第 15 週 | Protein-protein interaction, nucleic acid-protein interactions and immunoprecipitation & ChIP Metabolomics 12/23 |
| 第 16 週 | Flow cytometry 12/30 |
| 第 17 週 | 自主學習(課後報告) 1/6 |
| 第 18 週 | 自主學習(課後報告) 1/13 |
1. Molecular Cloning (4th ed., Michael R. Green and Joseph Sambrook) 2. Short Protocols in Cell Biology (1st ed., Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, and Kenneth M. Yamada)
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