顯微技術物理

Introduction and rules 課程簡介與規則 [Optical microscope 光學顯微鏡] Grouping 分組 Assignment 分配任務

[Optical microscope 光學顯微鏡] Microscope functions/structure: biological, metallurgical, stereo, upright, inverted Basic modes: transmission, reflection, bright field, dark field Special features: polarizing, fluorescence, phase contrast, working distance Key factors determining image resolution: - Numerical aperture - Abbe diffraction limit - Rayleigh criterion - Optical aberration - Nyquist Sampling Criterion 顯微鏡功能/結構：生物、金相、立體、正立、倒立 基本模式：穿透式、反射式、明視野、暗視野 特技：偏光、螢光、相位差、工作距離 決定影像解析度的關鍵因素： - 數值孔徑 Numerical aperture - 阿貝繞射極限 Abbe diffraction limit - 瑞利準則 Rayleigh criterion - 像差：光圈與像差的取捨 Optical aberration - 奈奎斯特取樣準則 Nyquist Sampling Criterion

[Optical microscope 光學顯微鏡] 製作樣本 Sample prep - 切片 slicing - 固定 fixing - 介質 medium

[Optical microscope 光學顯微鏡] 做實驗。 自由活動。 你可以在自己的研究實驗室做實驗。 也可以來教室用玩具顯微鏡練習基本技能。 Experiment day. You can do your experiment in your research lab. You can also come to the class room, use the demo microscope, practice the skills.

[Optical microscope 光學顯微鏡] 延續上週活動。 做實驗。 自由活動。 你可以在自己的研究實驗室做實驗。 也可以來教室用玩具顯微鏡練習基本技能。 Experiment day. You can do your experiment in your research lab. You can also come to the class room, use the demo microscope, practice the skills.

[Optical microscope 光學顯微鏡] 延續上週活動。 做實驗。 自由活動。 你可以在自己的研究實驗室做實驗。 也可以來教室用玩具顯微鏡練習基本技能。

[Optical microscope 光學顯微鏡] 成果發表會 Show case Review & wrap up

電子顯微鏡：介紹 Electron microscope: introduction Scanning electron microscope case study

Scanning electron microscope visiting

Scanning Tunneling Microscope case study

Scanning Tunneling Microscope visiting

Transmission Electron Microscope cases study

Transmission Electron Microscope visiting

Atomic Force Microscope case study

Atomic Force Microscope visiting

Final wrap up