光電生物物理技術
Optical and Electrical Techniques in Biophysics
| 節 | 週三 |
|---|---|
3 10:10–11:00 | 光電生物物理技術 BI301（博愛） 2 節連堂 |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
課程概述與目標： 概論光電元件於生物物理實用技術,並介紹如何將這些技術實際運用於現代分子和細胞生物物理和生物化學研究◦內容包括:幾何光學、光學元件原理與應用、常見用於生物物理實驗之電子儀器與應用實例◦ This course will provide fundamental concepts on optical and electrical techniques in biophysics. Those techniques are general used to study the structure and function of biological macromolecules. The goal of this course is to help students to develop physical understanding about daily-used instruments in biophysical and biochemistry laboratory.
基礎物理、基礎化學 (General physics and chemistry)
無備註
Lecture/ PowerPoint slides
In-class quiz(20% of the total final score) : 5 in-class quiz (MCQs) 作業(20% of the total final score) ：3~5 homework sets 作業繳交要求：可繳交紙本，亦可繳交電子檔並上傳於E3。選擇繳交電子檔，請掃描或拍照清楚，並整合為一個檔案，非壓縮檔。 考試 ( 60 % of the total final score)：one take-home midterm and one take-home final exam. Midterm: Lecture-knowledge based take-home midterm Final Exam: Article-based take-home exam
Light and Optics
1. Light and matter 2. Fundamental optics 3. Optical components 4. Light source: incoherent light and lasers
- 講授：
- 8
Electronics
1. Noise and response 2. Electronic circuit 3. Amplifier systems 4. Oscilloscope 5. Function generator
- 講授：
- 10
Detector and detections
1.Photomultilpier tubes 2. Photodiode 3. Avalance Photodiode 4. Photon counting 5. Charge-coupled Device 6. lock-in amplification
- 講授：
- 4
microscopy
1. Basic light microscopy, Confocal microscopy; Multi-photo fluorescent microscopy 2. Total internal reflection microscopy; Light-sheet illumination 3. Super-resolution microscopy 4. Penetration-depth improvement; CLARITY
- 講授：
- 8
- 實作：
- 2
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction Light and Matters Spectroscopy (Absorption) 2026-09-09(三) |
| 第 2 週 | Spectroscopy (Fluorescence) 2026-09-16(三) |
| 第 3 週 | Spectroscopy (IR and Raman) 2026-09-23(三) |
| 第 4 週 | Optical components I 2026-09-30(三) |
| 第 5 週 | Optical components II 2026-10-07(三) |
| 第 6 週 | Optical components III 2026-10-14(三) |
| 第 7 週 | Light source: incoherent light and lasers 2026-10-21(三) |
| 第 8 週 | Noise and response Detector and detection 2026-10-28(三) |
| 第 9 週 | Electronic circuit amplifier systems 2026-11-04(三) |
| 第 10 週 | Detector and detection Electronic circuit 2026-11-11(三) |
| 第 11 週 | Oscilloscope Function generator 2026-11-18(三) |
| 第 12 週 | Basic light microscopy, Confocal microscopy Multi-photo fluorescent microscopy 2026-11-25(三) |
| 第 13 週 | Total internal reflection microscopy Light-sheet illumination 2026-12-02(三) |
| 第 14 週 | Super-resolution microscopy 2026-12-09(三) |
| 第 15 週 | Penetration-depth improvement CLARITY 2026-12-16(三) |
| 第 16 週 | Case studies 2026-12-23(三) |
| 第 17 週 | Q&A for final exam 2026-12-30(三) |
| 第 18 週 | Q&A for final exam 2027-01-06(三) |
以下均為參考書目: ‧ John H. Moore, Christopher C. Davis, Michael A Coplan and Sandra C. Greer, Building scientific apparatus, 4th Ed., Cambridge, 2009 ‧Eugene Hecht, Optics, 4th Ed., Addison-Wesley, 2011 ‧ Joseph R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., Springer, 2010 ‧ Frank Trager ed., Spring handbook of lasers and optics, 2007
- 地點
- 生物科技學系 賢齊舘424室(博愛校區)
- 時間
- 週三 8:00~10:00
- 聯絡方式
- yatingkao@nycu.edu.tw