生物序列分析與高通量技術
Biological Sequence Analysis and High Throughput Technologies
| 節 | 週一 |
|---|---|
5 13:20–14:10 | 生物序列分析與高通量技術 3 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 |
* 根據陽明交大上課時間表所列
This class introduces the history of sequencing technologies in terms of their thermodynamic and chemical reaction principles and the applications of Next Generation Sequencing (NGS) with examples of the basic analysis. Several NGS strategies are covered, including whole genome sequencing, whole exome sequencing, RNA sequencing and metagenomics. Given that most of the NGS tools are Linux based, a taste of the Linux system will be given to students in the beginning of the course. Linux system and the scripting programming language R will be used in most of the examples.
基本分子生物學概念 (basic knowledge in molecular biological)
無備註
The class meets every Monday at 13:20 via the link below: Join Google Meet: https://meet.google.com/yzu-akfj-ocf
Midterm project (10%); Homework assignment (20%); in-class discussion & paper reading (40%); final project (oral presentation & Video Production) (30%)
Introduction
1.The descriptions and objectives of this course 2. Briefing on what and how you will learn from this course 3. Performance evaluations
- 講授：
- 3
Introduction of next generation sequencing (NGS)
1. Evolution of sequencing technology 2. NGS technology 3. 3rd generation sequencing
- 講授：
- 3
Whole genome sequencing
1. DNA sequencing 2. Single nucleotide variation 3. Copy number variation
- 講授：
- 2
- 示範：
- 0.5
- 實作：
- 0.5
Target sequencing
1. Single nucleotide variation 2. Copy number variation
- 講授：
- 2
- 示範：
- 0.5
- 實作：
- 0.5
參訪生物科技公司
- 講授：
- 0
- 示範：
- 3
Transcriptome profiling & functional analysis
1. RNA sequencing 2. Expression microarray 3. Transcriptome quantification 4. Differentially expressed gene identification 5. KEGG, GO, GSEA
- 講授：
- 4
- 示範：
- 1
- 實作：
- 1
Small RNA Sequencing
1. What’s miRNA 2. miRNA mRNA regulatory network
- 講授：
- 2
- 示範：
- 0.5
- 實作：
- 0.5
Bacterial genome assembly
1. de novo assembly 2. 3rd generation sequencing
- 講授：
- 2
- 示範：
- 0.5
- 實作：
- 0.5
Introduction of Linux
1. Linux system 2. less, more, cat … 3. Obtain data from website
- 講授：
- 4
- 示範：
- 1
- 實作：
- 1
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction 2026-09-07(一) |
| 第 2 週 | Introduction of Next Generation Sequencing (NGS) 2026-09-14(一) |
| 第 3 週 | 中秋節(放假) 2026-09-21(一) |
| 第 4 週 | Whole genome sequencing 2026-09-28(一) |
| 第 5 週 | Target sequencing (Whole exome sequencing & disease panels) 2026-10-05(一) |
| 第 6 週 | Transcriptome profiling & functional analysis (I) 2026-10-12(一) |
| 第 7 週 | Transcriptome profiling & functional analysis (II) 2026-10-19(一) |
| 第 8 週 | 光復節補假 2026-10-26(一) |
| 第 9 週 | Small RNA Sequencing 2026-11-02(一) |
| 第 10 週 | Introduction of Linux (I) 2026-11-09(一) |
| 第 11 週 | Introduction of Linux (II) 2026-11-16(一) |
| 第 12 週 | An industry visit to a biotechnology company 2026-11-23(一) |
| 第 13 週 | Bacterial genome assembly (NGS & 3rd generation sequencing) 2026-11-30(一) |
| 第 14 週 | non model organism, microbiome 2026-12-07(一) |
| 第 15 週 | Final project rehearsal (I) 2026-12-14(一) |
| 第 16 週 | Final project rehearsal (II) 2026-12-21(一) |
教師未提供此項資料
- 地點
- BIO-ICT building R420
- 時間
- Fri 9:00-10:00
- 聯絡方式
- 03-5712121 ext. 59734