微生物基因工程
Microbial Genetic Engineering
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 微生物基因工程 BI301（博愛） 3 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 |
* 根據陽明交大上課時間表所列
Class Description: “Microbial Genetic Engineering " is a comprehensive, hands-on course designed to guide students through the fundamental techniques employed in the rapidly evolving fields of Genetic Engineering. The course covers a range of topics including plasmid construction, gene expression manipulation, bacterial host genomic modifications, enzyme bioprospecting, enzyme assay and pathway assay, protein engineering and direct evolution, and an introduction to the latest sequencing technologies. Through real-world examples, students will learn how to design, conduct, and interpret experimental results, and gain a deeper understanding of the practical aspects of these technologies. Purpose of the Class: The purpose of this course is to equip students with the practical skills and knowledge in Genetic Engineering, which are essential for contributing to research and development in the fields of Metabolic Engineering and Synthetic Biology. It aims to give students a solid foundation of key techniques, encourage critical thinking, promote innovative experimental design, and foster an understanding of the practical applications of these concepts in various applications, including biotechnology, biofuel and biochemical productions, and pharmaceuticals. This class will be taught in English.
Molecular biology (I), Biochemistry (I) & (II), and/or Microbiology, are recommended.
無備註
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Final exam : 30 % Assignments: 30 % Presentation: 25 % Class participation: 15 %
教師未提供此項資料
| 週次 | 主題 |
|---|---|
| 第 1 週 | Course Introduction and Overview 2026-09-11(五) 時數：[2026-09-11]林柏亨(3.00) |
| 第 2 週 | Introduction of Expression Systems: promoter, RBS and plasmid origins 2026-09-18(五) 時數：[2026-09-18]林柏亨(3.00) |
| 第 3 週 | Holidays 2026-09-25(五) |
| 第 4 週 | Steps by Steps for Plasmid Constructions (with introduction of software and online tools) 2026-10-02(五) 時數：[2026-10-02]林柏亨(3.00) |
| 第 5 週 | Holidays 2026-10-09(五) |
| 第 6 週 | Case Study: Promoter prospecting in Geobacillus 2026-10-16(五) 時數：[2026-10-16]林柏亨(3.00) |
| 第 7 週 | Case Study: Enzyme Bioprospecting Using Kivd as an example 2026-10-23(五) 時數：[2026-10-23]林柏亨(3.00) |
| 第 8 週 | Case Study: Enzyme Assay & Pathway Assay Using Kivd, Adh as example for Enzyme Assay Using rGPS-MCG pathway as an example for Pathway Assay 2026-10-30(五) 時數：[2026-10-30]林柏亨(3.00) |
| 第 9 週 | Protein Engineering & Direct Evolution. Case Study: Protein Engineering & Direct Evolution Using Kivd and Mdh as examples 2026-11-06(五) 時數：[2026-11-06]林柏亨(3.00) |
| 第 10 週 | Bacterial Host Genomic Modifications. 2026-11-13(五) 時數：[2026-11-13]林柏亨(3.00) |
| 第 11 週 | Case Study: Bacterial Host Genomic Modifications – Gene Deletion (with Assignments) (Using ptsG deletion for lambda-Red system, lsrF deletion for CRISPR system) 2026-11-20(五) 時數：[2026-11-20]林柏亨(3.00) |
| 第 12 週 | Case Study: Bacterial Host Genomic Modifications – Gene Integration (Using xpk integration CRISPR and Transposon-encoded CRISPR–Cas systems) 2026-11-27(五) 時數：[2026-11-27]林柏亨(3.00) |
| 第 13 週 | Sanger Sequence Applications, NGS & Nanopore Applications 2026-12-04(五) 時數：[2026-12-04]林柏亨(3.00) |
| 第 14 週 | Literature presentation and discussion 2026-12-11(五) 時數：[2026-12-11]林柏亨(3.00) |
| 第 15 週 | Final exam week 2026-12-18(五) 時數：[2026-12-18]林柏亨(3.00) |
| 第 16 週 | Holidays 2026-12-25(五) |
Primary material: Lecture notes
- 地點
- BioICT Building (博愛校區賢齊館) Rm519
- 時間
- By appointment
- 聯絡方式
- Email: linp@nycu.edu.tw